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Polyamines and polyamine complexes: analytical comments

D Bartos, F Bartos, R A Campbell

    Physiological Chemistry and Physics
    |January 1, 1980
    PubMed
    Summary
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    This study discusses methods for measuring polyamines in biological samples. Hydrolysis of samples reveals higher polyamine levels, indicating conjugates and protein-bound forms are present.

    Area of Science:

    • Biochemistry
    • Clinical Chemistry
    • Analytical Chemistry

    Background:

    • Di- and polyamines (putrescine, spermidine, spermine) are crucial biomarkers.
    • Their accurate quantification in biological fluids like serum, plasma, and urine is vital for health and disease studies.
    • Understanding polyamine conjugates and protein-bound forms is essential for comprehensive analysis.

    Purpose of the Study:

    • To review and discuss sample preparation methods for determining di- and polyamines and their conjugates.
    • To highlight the significance of hydrolysis in revealing total polyamine content.
    • To suggest potential analytical techniques for future biological studies.

    Main Methods:

    • Discussion of various sample preparation techniques for serum, plasma, and urine.

    Related Experiment Videos

  • Analysis of polyamine levels before and after acid hydrolysis.
  • Consideration of radioimmunoassays for conjugate measurements.

    • Main Results:

    • Hydrolysis of acidic extracts from serum or plasma yields higher polyamine values.
    • This suggests the presence of polyamine conjugates in these samples.
    • Polyamines may also be bound to proteins, requiring release via hydrolysis.

    Conclusions:

    • Accurate determination of di- and polyamines requires effective sample preparation, including hydrolysis.
    • The presence of conjugates and protein-bound polyamines necessitates specific analytical approaches.
    • Radioimmunoassays using antibodies against polyamine conjugates could be valuable for future quantitative studies.