Lipoprotein uptake in perfused arteries: inhibition by 7-ketocholesterol

The effect of 7-Ketocholesterol (7-KC), an oxygenated sterol (OS) on the uptake of low density lipoproteins (LDL) by artificially perfused porcine carotid arteries was studied. The perfusate consisted of Dulbecco's modified Eagle medium, supplemented with albumin and pure 125I-LDL. Prior exposure to 7-KC significantly inhibited LDL uptake (from 2.47 to 1.92 micrograms LDL p/gr artery). The degree of inhibition of LDL uptake was less than that previously observed for arterial influx of cholesterol.