Topochemical variation of LDH and CK isoenzyme patterns in aorta

LDH and CK isoenzyme patterns are determined electrophoretically (Beckman) from various aortic regions and layers and in atherosclerotic plaques as compared to control areas. Throughout consecutive layers of human aorta LDH isoenzymes show a continuous cathodic shift from both surfaces towards the center with a maximum between the inner and middle third. CK shows a heterogenous pattern with maxima at CK MM and CK BB and less CK MB. Most of the CK MM is located in the intima, the media contains more of CK BB. Regional analysis shows an anodic shift of CK from proximal to distal thoracic aorta. In experimental short term hypertension a definite anodic CK isoenzyme shift is observed with increased relative CK BB activity.