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Measurement of functional C2--a method for clinical laboratories
Journal of Immunological Methods
|January 1, 1978
Summary
This study presents a straightforward method to measure functional complement component 2 (C2) in human serum. The assay utilizes common laboratory reagents for accessible complement system analysis.
Area of Science:
- Immunology
- Biochemistry
- Complement System Research
Background:
- The complement system is crucial for innate immunity.
- Functional assays are vital for assessing complement pathway activity.
- Complement component 2 (C2) plays a key role in complement activation.
Purpose of the Study:
- To describe a practical method for quantifying functional C2 in human serum.
- To enable accessible assessment of the classical and lectin complement pathways.
Main Methods:
- Utilized sensitized sheep erythrocytes as indicator cells.
- Employed zymosan-treated normal human serum as a complement source.
- Incorporated guinea pig serum as a source of complement factors.
- Developed a functional assay for C2 measurement.
Main Results:
- Successfully established a method for measuring functional C2.
- Demonstrated the utility of readily available reagents for the assay.
- Provided a basis for further studies on complement deficiencies.
Conclusions:
- The described method offers a reliable and accessible approach to assess functional C2.
- This assay can be valuable in clinical and research settings for evaluating complement function.