Blood preservation 35. Red cell 2,3-DPG and ATP maintained by DHA-ascorbate-phosphate

|March 1, 1981

Summary

Dihydroxyacetone (DHA) with ascorbic acid maintains normal 2,3-DPG levels for six weeks in blood preservation. Phosphate addition modifies DHA

Area of Science:

Background:

Maintaining 2,3-diphosphoglycerate (2,3-DPG) levels is crucial for red blood cell (RBC) function during storage.
DHA and ascorbic acid are investigated as potential additives for blood preservation.
Phosphate's role in modulating 2,3-DPG and ATP levels in stored RBCs requires further elucidation.

Purpose of the Study:

To evaluate the efficacy of dihydroxyacetone (DHA) and ascorbic acid, alone and in combination with phosphate, on maintaining 2,3-DPG and ATP levels in stored red blood cells.
To determine the optimal concentrations and combinations of these additives for extended blood preservation.

Main Methods:

Red blood cells were stored with various concentrations of DHA (60 mM), ascorbic acid (10 mM), and phosphate.
2,3-DPG and ATP levels were measured at different time points (e.g., two, four, and six weeks).
Comparison with a standard CPD-adenine preservative was performed.

Main Results:

DHA (60 mM) combined with ascorbic acid (10 mM) maintained normal 2,3-DPG levels for six weeks.
DHA alone led to subnormal 2,3-DPG after four weeks; DHA-ascorbate-phosphate resulted in subnormal levels after two weeks.
High phosphate concentrations negatively impacted 2,3-DPG maintenance.
ATP maintenance was optimal with DHA-phosphate (20 mM), followed by DHA-phosphate (10 mM), CPD-adenine, Phosphate (20 mM), and DHA.
10 mM phosphate mitigated the adverse effects of DHA and DHA-ascorbate on ATP levels.

Conclusions:

Dihydroxyacetone (DHA) and ascorbic acid combination is effective in preserving 2,3-DPG levels for up to six weeks.
Phosphate supplementation, particularly at high concentrations, can impair 2,3-DPG maintenance but is crucial for optimal ATP levels when combined with DHA.
The combination of DHA and phosphate offers a promising strategy for improving red blood cell preservation by balancing 2,3-DPG and ATP stability.

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