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Published on: November 12, 2015
In vitro growth of some fastidious adenoviruses from stool specimens
Abstract:
Sixty-seven stool specimens from 51 children, positive for adenoviruses by electron microscopy and negative for growth in human-embryo kidney cells, were tested for growth in Chang conjunctiva cells. Twenty-eight specimens caused a cytopathic effect over more than one passage in these cultures, and several adenovirus strains grew better at 33 degree C than at 37 degree C. Most of the culture-positive specimens also induced the development of adenovirus antigens in KB cells detectable by a group-specific indirect immunofluorescence test. Twenty-four of the 25 fastidious strains tested were antigenically related and were distinct from the established serotypes commonly isolated from stools.
Insights
Researchers identified fastidious adenovirus strains in children's stool samples. These novel strains, distinct from common types, grew better in specific cell cultures at a lower temperature.
Area of Science:
- Virology
- Pediatric Infectious Diseases
Background:
- Adenoviruses are common viral pathogens in children.
- Some adenovirus strains are difficult to culture using standard methods.
- Electron microscopy can detect adenoviruses but doesn't confirm infectivity.
Purpose of the Study:
- To isolate and characterize fastidious adenovirus strains from pediatric stool samples.
- To evaluate the efficacy of Chang conjunctiva cells for adenovirus isolation.
- To determine the antigenic relationship of newly isolated strains.
Main Methods:
- Stool specimens from children positive for adenoviruses by electron microscopy were cultured.
- Chang conjunctiva cells were used for viral isolation and propagation.
- Cytopathic effects and viral antigen presence (immunofluorescence) were assessed.
- Temperature-dependent growth characteristics were investigated.
- Antigenic relatedness of isolates was determined.
Main Results:
- Twenty-eight of 67 specimens yielded adenovirus growth in Chang conjunctiva cells after initial electron microscopy detection.
- Several isolated adenovirus strains exhibited enhanced growth at 33°C compared to 37°C.
- Immunofluorescence assays confirmed adenovirus antigen presence in culture-positive samples.
- Twenty-four of 25 fastidious strains were antigenically related and distinct from known stool adenovirus serotypes.
Conclusions:
- Chang conjunctiva cells are effective for isolating fastidious adenoviruses from pediatric stool.
- A subset of pediatric adenoviruses shows temperature-dependent growth preferences.
- A novel, antigenically distinct group of adenoviruses circulates in children.
