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Sugar absorption and secretion by winter flounder intestine
The American Journal of Physiology
|May 1, 1981
Summary
Winter flounder intestine exhibits two active sugar transport systems. One system absorbs sugars, while another secretes them, leading to no net D-galactose transport due to dual system involvement.
Area of Science:
- Physiology
- Marine Biology
- Biochemistry
Background:
- The intestine plays a crucial role in nutrient absorption.
- Understanding sugar transport mechanisms is vital for fish physiology.
Purpose of the Study:
- To investigate sugar transport mechanisms in winter flounder intestine.
- To identify and characterize active sugar transport systems.
Main Methods:
- Experiments involved measuring sugar fluxes across the intestinal tissue.
- Inhibitors like phlorizin, ouabain, and phloretin were used.
- Substitutions of sodium (Na+) with choline were performed.
Main Results:
- Two distinct Na+-dependent sugar transport systems were identified: one for absorption and one for secretion.
- D-galactose transport was affected by both systems, resulting in no net flux.
- Net secretion of 2-deoxy-D-galactose was observed, with specific kinetic properties and inhibitors.
Conclusions:
- Winter flounder intestine possesses at least two active sugar transport systems.
- These systems operate across the mucosal border, influencing net sugar transport.
- The interaction of D-galactose with these systems explains its lack of net flux.