[Conformation of cytochrome b5 in soluble form and incorporated into artificial membranes]

It was shown that 35% of the peptide groups, which form the right alpha-helices, are presented in the soluble fraction of cytochrome b5. The incorporation of cytochrome b5 into egg lecithine liposomes increases the number of alpha-helices up to 51%. On the contrary, the cytochrome incorporation into microsomal lipid liposomes slightly increases the degree of spiralization. The reduction of cytochrome b5 haem slightly decreases alpha-helices in the soluble haemoprotein as well as in the one incorporated into the artificial membrane irrespective of its phospholipid content.