Related Experiment Video
Updated: May 5, 2026

High-resolution Imaging and Analysis of Individual Astral Microtubule Dynamics in Budding Yeast
Published on: April 20, 2017
Molecular analysis of cytoplasmic microtubules in situ: identification of both widespread and specific proteins
Abstract:
We have analyzed the molecular components of cytoplasmic microtubules in situ in several types of cultured cells. Our approach makes use of preparations of detergent-extracted cytoskeletons that contain the original cellular microtubules. Identification of microtubule components is based on two criteria: their presence in the cytoskeleton preparations is dependent upon the presence of intact microtubules, and they are released from such preparations by buffers that depolymerize microtubules. Proteins that meet these criteria are described. Some of them are restricted to cells of certain species or tissues of origin. Others appear to be of widespread distribution. Among the latter is a protein homologous to one of the tau polypeptides in brain microtubule protein.
More Related Videos
08:44Visualizing Actin and Microtubule Coupling Dynamics In Vitro by Total Internal Reflection Fluorescence TIRF Microscopy
Published on: July 20, 2022
08:02Extracting Modified Microtubules from Mammalian Cells to Study Microtubule-Protein Complexes by Cryo-Electron Microscopy
Published on: March 3, 2023
Related Concept Videos
Microtubules
Assembly of Cytoskeletal Filaments
Microtubules
Microtubules have two structurally similar globular protein subunits: α and β tubulins. In the cytosol, the α and β tubulins form a heterodimer....
Microtubule Formation
Microtubule Associated Proteins (MAPs)
Microtubule Associated Motor Proteins