Radioimmunoassay of T-2 toxin in biological fluids

A simple radioimmunoassay (RIA) for detection of T-2 toxin in biological fluids was developed. The method, which involves a Sep-Pak C18 reverse phase column cleanup step before RIA, can detect 0.5 ppb T-2 toxin in serum and 2.5 ppb T-2 toxin in urine and milk. Recoveries for toxin added to serum and urine or milk samples were 87-106% in the 0.5-20 ppb range and 88-117% in the 2.5-20ppb range, respectively. If samples were not cleaned up, false positive results prevented positive detection at concentration less than 2.5 ppb in urine and milk.