A rapid microchromatographic method for determination of hemoglobin AI

Area of Science:

• Clinical Chemistry
• Endocrinology
• Hematology

Background:

• Hemoglobin AI (HbAI) is a minor hemoglobin fraction formed by erythrocyte glycosylation.
• HbAI concentration correlates with average blood glucose levels over the preceding 1-2 months.
• HbAI determination is a valuable tool for assessing long-term diabetes control.

Purpose of the Study:

• To describe a simple, reliable microchromatographic method for HbAI determination.
• To evaluate the suitability of this method for routine clinical analysis.
• To compare the developed method with a commercial kit.

Main Methods:

• Development of a microchromatographic technique for HbAI quantification.
• Analysis of HbAI levels in diabetic patients (n=126) and a reference group (n=33).
• Comparative analysis with a commercial Bio-Rad HbAI kit.

Main Results:

• The developed microchromatographic method is simple, reliable, and suitable for routine clinical use.
• Mean HbAI values were 9.6 ± 1.8% in diabetics and 6.0 ± 0.8% in the reference group.
• The developed method demonstrated equal accuracy and comparable results to the commercial Bio-Rad kit.

Conclusions:

• The described microchromatographic method provides an accurate and reliable means for HbAI determination.
• This method is well-suited for routine clinical analysis in diabetes management.
• The new method is a viable alternative to existing commercial kits.