Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Blinded proficiency testing of FTA-ABS test

G L Goodhart, S T Brown, A A Zaidi

    Archives of Internal Medicine
    |July 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Strawberry latent ringspot virus Infecting Roses in India.

    Plant disease·2019
    Same author

    Occurrence of Cucumber mosaic virus in Gerbera jamesonii in India.

    Plant disease·2019
    Same author

    Natural Occurrence of a Potyvirus on Murraya koenigii in India.

    Plant disease·2019
    Same author

    Wild Himalayan Cherry (Prunus cerasoides) as a Natural Host of Prunus necrotic ringspot virus in India.

    Plant disease·2019
    Same author

    A simplified strategy for studying the etiology of viral diseases: Apple stem grooving virus as a case study.

    Journal of virological methods·2014
    Same author

    In Vitro Expression and Production of Antibody Against Cymbidium mosaic virus Coat Protein.

    Indian journal of virology : an official organ of Indian Virological Society·2013

    Proficiency testing for syphilis serologic tests showed VDRL and FTA-ABS tests had similar reproducibility. High-quality laboratory performance is crucial for accurate syphilis diagnosis today.

    Area of Science:

    • Clinical Laboratory Science
    • Infectious Disease Diagnostics
    • Public Health Surveillance

    Background:

    • Syphilis diagnosis relies heavily on serologic testing.
    • Proficiency testing is essential for maintaining laboratory accuracy.
    • Evaluating the performance of standard syphilis serologic assays is critical.

    Purpose of the Study:

    • To assess the proficiency of state laboratories in performing syphilis serologic tests.
    • To compare the reproducibility of the VDRL and FTA-ABS tests.
    • To evaluate the impact of laboratory proficiency on diagnostic accuracy in low-prevalence settings.

    Main Methods:

    • A blinded proficiency testing study involving 28 state laboratories.
    • Utilized a battery of 50 duplicate reagin-positive sera, including 12 from syphilitic patients.

    Related Experiment Videos

  • Analyzed reproducibility scores for the Venereal Disease Research Laboratory (VDRL) and Fluorescent Treponemal Antibody Absorption (FTA-ABS) tests.

    • Main Results:

    • The median reproducibility scores for both VDRL and FTA-ABS tests were identical at 92%.
    • Reproducibility scores between the two tests demonstrated a positive correlation.
    • High laboratory proficiency is necessary for reliable syphilis serologic results in low-prevalence populations.

    Conclusions:

    • The VDRL and FTA-ABS tests exhibit comparable reproducibility in proficiency testing.
    • Accurate syphilis diagnosis in the current low-prevalence environment depends on highly proficient laboratories.
    • Physicians should integrate clinical judgment with serologic testing for optimal patient management.