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Hair acid phosphatase: multiple forms, glycoprotein nature and characterization
Enzyme
|January 1, 1981
Summary
Guinea pig hair acid phosphatase exists in multiple forms and is a glycoprotein. Purification revealed properties similar to lysosomal acid phosphatase found in other organs.
Area of Science:
- Biochemistry
- Enzymology
- Glycobiology
Background:
- Acid phosphatase is an enzyme found in various tissues.
- Guinea pig hair contains acid phosphatase, but its nature is not fully characterized.
- Understanding enzyme properties aids in diagnosing and treating related conditions.
Purpose of the Study:
- To investigate the biochemical properties of guinea pig hair acid phosphatase.
- To determine if hair acid phosphatase is a glycoprotein.
- To compare its characteristics with lysosomal acid phosphatase from other organs.
Main Methods:
- Isoelectric focusing and electrophoresis to analyze enzyme forms.
- Concanavalin-A-Sepharose chromatography to assess glycoprotein nature.
- DEAE-cellulose, CM-cellulose chromatography, and Sephadex G-100 gel filtration for purification.
Main Results:
- Multiple electrophoretic forms of guinea pig hair acid phosphatase were identified.
- The enzyme demonstrated binding to concanavalin-A-Sepharose, with 60% eluted by alpha-methyl-D-glucoside, indicating it is a glycoprotein.
- Purified enzyme exhibited properties similar to lysosomal acid phosphatase from other organs.
Conclusions:
- Guinea pig hair acid phosphatase is a glycoprotein present in multiple forms.
- The enzyme shares biochemical similarities with lysosomal acid phosphatase.
- These findings contribute to understanding the role and nature of hair-associated enzymes.