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Confocal Imaging of Double-Stranded RNA and Pattern Recognition Receptors in Negative-Sense RNA Virus Infection
Published on: January 26, 2019
Does rice ragged stunt virus lack the typical double shell of the Reoviridae?
Abstract:
In rice ragged stunt virus preparations negatively stained with uranyl acetate, particles corresponding to the B-spiked subviral particles (SPVs) but not the double-shelled virions of fijiviruses were seen. The B spikes were broader at the base than those of fijiviruses, but otherwise the particles were similar in size and shape to fijivirus SVPs. In thin sections of infected plants, large numbers of virus-like particles were seen; most resembled the SVPs of fijiviruses but some appeared intermediate between these and complete fijivirus particles. It seems likely that rice ragged stunt virus does not produce a regular outer shell as do the fijiviruses, although the broad-based B spikes may in some profiles have given the appearance of a continuous shell.
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