Statistically designed assay of migration-inhibitory activity in lymphokine preparations

The migration inhibiton test in agarose as described by Clausen (Acta Allergol 26:56, 1971) was modified into a statistically designed assay comprising a laboratory standard in each assay, and using buffy coat cells or peripheral polymorphonuclear leukocytes from pigs as target cells. The precision of the assay was improved by combining cells from several pigs. Fifty-5000 microliters of lymphokine-containing test fluid could be used in the assay provided that this fluid contains the essential nutrients. In our hands, the capillary tests was unsuitable for a quantitative assessment of migration inhibition activity.