High-performance liquid chromatographic measurement of cloprednol in human plasma

A rapid and specific high-performance liquid chromatographic method for the quantitative determination of cloprednol in human plasma is described. Samples were extracted using methylene chloride-ether (40:60) and then purified further by solvent and pH partitioning techniques. Cloprednol was analyzed using normal-phase chromatography and UV detection at 254 nm. The final recovery after losses during the cleanup procedure for cloprednol from human plasma was 80.8%. The lowest concentration that could be measured with confidence was 8 ng/ml.