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Updated: Aug 12, 2026

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
Published on: April 3, 2014
Inhibition of terminal deoxynucleotidyltransferase by various diadenosine polyphosphates
Abstract:
Terminal deoxynucleotidyltransferase (TdT) was found to be strongly inhibited by diadenosine 5',5"'-P1,P4-tetraphosphate (AP4A) while other mammalian DNA polymerases alpha, beta and gamma were neither inhibited nor activated by AP4A. Such inhibitory effect on TdT was also observed with a variety of diadenosine polyphosphates (APnA), n = 3-6) when TdT activity was assayed by determining incorporation of dCTP. Kinetic analysis revealed that the inhibition of TdT by APnA was due to competition with substrate deoxynucleoside triphosphate(s).
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