Partial rat kidney resection using autologous fibrinogen thrombin adhesive system

Area of Science:

• Biomedical Engineering
• Surgical Innovation
• Regenerative Medicine

Background:

• Local hemostasis in parenchymatous organs is critical for minimizing tissue damage during surgery.
• The Fibrinogen Thrombin Adhesive System (FTAS) is designed to provide effective local hemostasis.
• Understanding the degradation and integration of hemostatic agents is crucial for assessing their safety and efficacy.

Purpose of the Study:

• To investigate the degradation profile of the Fibrinogen Thrombin Adhesive System (FTAS) in vivo.
• To evaluate the process of wound healing following partial kidney resection using FTAS for hemostasis.
• To assess the biocompatibility and safety of FTAS in renal surgery.

Main Methods:

• Partial kidney resection was performed in rats, with hemostasis achieved using FTAS.
• Experimental groups included animals treated with labeled (125I) and unlabeled FTAS.
• Radioactive iodine (125I) excretion was measured, and kidneys were analyzed via light and electron microscopy and autoradiography.

Main Results:

• Two peaks of 125I excretion were observed, indicating initial free iodine release and subsequent FTAS degradation around 120 hours post-application.
• No fibrinolysis was observed, and FTAS was primarily resorbed by macrophages.
• Wound healing progression correlated with physiological fibrinogen concentration, and no renal parenchymal damage was detected.

Conclusions:

• FTAS facilitates effective local hemostasis in renal surgery without causing tissue damage.
• The degradation of FTAS occurs primarily through macrophage-mediated resorption, without inducing fibrinolysis.
• The findings support the safety and efficacy of FTAS for promoting wound healing in kidney surgery.