Related Experiment Video
Updated: Aug 16, 2026

Milk Collection in the Rat Using Capillary Tubes and Estimation of Milk Fat Content by Creamatocrit
Published on: December 16, 2015
Prolactin content in rat pituitary gland. RIA of prolactin after different extraction procedures
Abstract:
The prolactin content of rat pituitary varies considerably when determined by RIA, due to incomplete solubilization of prolactin secretory granules with standard procedures for tissue homogenization and centrifugation. Freezing and thawing, Triton X-100 and ultrasonic treatments increased the yield of prolactin significantly but electron microscopy of pellets revealed numerous unmodified secretory granules. Addition of 2.5 M urea produced complete extraction of tissue prolactin confirmed by RIA of supernatants and electron microscopy of pellets.
More Related Videos
09:12Assessing Cellular Stress and Inflammation in Discrete Oxytocin-secreting Brain Nuclei in the Neonatal Rat Before and After First Colostrum Feeding
Published on: November 14, 2018
12:57X-Ray Visualization of Intraductal Ethanol-Based Ablative Treatment for Prevention of Breast Cancer in Rat Models
Published on: December 9, 2022