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Genetic transformation in Streptococcus sanguis. Competence factor and competence factor inactivator
Acta Pathologica Et Microbiologica Scandinavica. Section B, Microbiology
|April 1, 1981
Summary
Genetic transformation in Streptococcus sanguis relies on competence factor (CF). A newly identified competence factor inactivator (CFI) impacts CF stability, suggesting a protein with enzymatic activity.
Area of Science:
- Microbiology
- Molecular Biology
- Bacteriology
Background:
- Streptococcus sanguis genetic transformation is a complex process.
- Competence factor (CF) in culture filtrates is essential for transformation.
- Other factors influence CF activity and stability.
Purpose of the Study:
- To investigate factors affecting competence factor (CF) stability in Streptococcus sanguis.
- To identify and characterize a novel factor influencing CF activity.
Main Methods:
- Preparation of culture filtrates from Streptococcus sanguis strains.
- Assessing competence factor (CF) activity and stability under various conditions.
- Heat inactivation and enzymatic digestion to characterize the inactivator.
Main Results:
- A competence factor inactivator (CFI) was identified, affecting CF stability.
- CFI is thermolabile, inactivated by heating at 65°C for 15 minutes.
- CFs from specific strains (Challis, 13b) are heat-stable and resist inactivation.
Conclusions:
- A competence factor inactivator (CFI) plays a role in regulating genetic transformation in Streptococcus sanguis.
- CFI may be a protein with enzymatic activity, impacting CF stability.
- Understanding CFI is crucial for optimizing genetic transformation protocols in streptococci.