[Study of the hydrodynamic properties of solutions of T2 phage DNA using a rotation viscosimeter]
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Biofizika
|March 1, 1981
Abstract:
Viscosity of DNA solutions of phage T2 at different rate gradients was investigated in relation to ionic strength and solution temperature. Measurements were performed by means of rotation viscosimeter with a magnetic support of the rotor. The slope of gradient relationship was shown to increase with a decrease of ionic strength of DNA solution. A significant temperature relationship of solution viscosity at T less than Tm was observed. A slope of the curves eta sp = f (T) depends on DNA concentration in solution. It was shown that in the presence of Cu+2 or Mg+2 ions at a cyclic change of temperature a small irreversible change of DNA solution viscosity was observed.