[Effect of cyclic nucleotides and histone fractions on mitochondrial respiratory and phosphorylating activity]

CAMP in the wide range of concentrations (3.10(-7)--10(-3) M), cGMP (10(-6)--10(-5) M) and dibutyril-cAMP (3.10(-6) M) produced no effect on oxidative phosphorylation of rat liver mitochondria. Among histone fractions (H1, H3 and H4) tested, the arginine-rich fraction H3 (18 microgram/mg of mitochondrial protein) produced a strong uncoupling effect. It is supposed that the effect of cAMP on oxidative phosphorylation of mitochondria may be carried out indirectly through activation of protein kinase, phosphorylation of histones, and their release into the cytoplasm.