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Related Experiment Videos

Automated in-line ratio-correcting filter fluorometer

O S Khalil, W S Routh, K Lingenfelter

    Clinical Chemistry
    |September 1, 1981
    PubMed
    Summary
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    A new optical setup transforms the Abbott VP analyzer into a fluorometer, enabling fluorescence measurements with high sensitivity. This modification offers a cost-effective way to expand analytical capabilities without major hardware changes.

    Area of Science:

    • Biomedical Engineering
    • Analytical Chemistry
    • Optical Physics

    Background:

    • The Abbott VP bichromatic analyzer is a widely used instrument for absorbance-based assays.
    • Expanding its functionality to fluorescence detection can broaden its applications in clinical diagnostics and research.
    • Existing methods for fluorescence analysis often require dedicated instruments or significant hardware modifications.

    Purpose of the Study:

    • To develop a novel optical arrangement for the Abbott VP bichromatic analyzer to enable fluorescence measurements.
    • To create a cost-effective and easily integrated fluorometric capability for the existing analyzer.
    • To validate the modified system's performance for clinical and biochemical assays.

    Main Methods:

    • A specially designed filter carriage was inserted into the Abbott VP analyzer to convert it into a fluorometer.

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  • Straight-through excitation geometry was employed for fluorescence intensity measurement.
  • Light-source intensity fluctuations were corrected using the attenuated transmitted beam as a reference.
  • The modified system was tested for detection limits using fluorescein and applied to a fluorescence immunoassay for theophylline.

    • Main Results:

    • The modified Abbott VP analyzer demonstrated a detection limit of 192 pmol/L for fluorescein.
    • Fluorescence immunoassay data for theophylline in patient sera showed excellent correlation (r = 0.969) with results from an enzyme immunoassay (EMIT).
    • A fluorometric standard curve for glucose was successfully generated, indicating the system's versatility.

    Conclusions:

    • The novel optical arrangement effectively converts the Abbott VP bichromatic analyzer into a functional fluorometer.
    • This modification provides a simple, economical method to enhance the analyzer's capabilities without auxiliary light sources or major hardware changes.
    • The validated performance suggests the modified system is suitable for various fluorescence-based assays, including immunoassays and biochemical analyses.