Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Increased nucleotide catabolism after cerebral convulsions

H Manzke, W Staemmler, K Dörner

    Neuropediatrics
    |May 1, 1981
    PubMed
    Summary

    Oxypurine levels in cerebrospinal fluid (CSF) significantly increase after seizures in both rabbits and children. Elevated hypoxanthine and uric acid in children suggest a link between convulsions and these biomarkers.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Myofascial chronic pelvic pain in women : A retrospective evaluation of the effects of specific diagnostics and therapy].

    Schmerz (Berlin, Germany)·2020
    Same author

    A versatile liquid-jet setup for the European XFEL.

    Journal of synchrotron radiation·2019
    Same author

    AXSIS: Exploring the frontiers in attosecond X-ray science, imaging and spectroscopy.

    Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment·2017
    Same author

    [Visions about a new system of health care].

    Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz·2008
    Same author

    [The new human mode of being demented].

    Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz·2005
    Same author

    Molybdenum metabolism: stable isotope studies in infancy.

    Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS)·2002

    Area of Science:

    • Neurochemistry
    • Biomarkers of Seizures
    • Convulsive Disorders

    Background:

    • Oxypurines are breakdown products of nucleic acids.
    • Seizures cause significant metabolic changes in the brain.
    • Identifying reliable biomarkers for seizure activity is crucial for diagnosis and management.

    Purpose of the Study:

    • To investigate the temporal changes in oxypurine concentrations in cerebrospinal fluid (CSF) following induced seizures in rabbits.
    • To evaluate the diagnostic potential of hypoxanthine and uric acid as biomarkers in children with various seizure types and neurological conditions.

    Main Methods:

    • Cardiazol-induced seizures in rabbits were used to analyze CSF and serum oxypurine, creatinine, and potassium levels over 24 hours.
    • HPLC, densitometric thin-layer, and enzymatic-oxymetric methods were employed for quantification.
    • CSF samples from 31 children with seizures and 20 children with aseptic meningitis were analyzed for hypoxanthine and uric acid concentrations.

    Main Results:

    • In rabbits, CSF oxypurine concentrations peaked at 1 hour post-seizure, remaining elevated for 24 hours.
    • Creatinine and potassium were transiently elevated post-seizure in rabbits.
    • In children, elevated hypoxanthine was observed in severe grand mal seizures and petit mal status, with significantly higher frequencies compared to aseptic meningitis (p < 0.005).
    • Raised uric acid was also more frequent in children with convulsions (70%) versus aseptic meningitis (40%) (p < 0.05).

    Conclusions:

    • Oxypurines, particularly hypoxanthine, show promise as sensitive biomarkers for seizure activity in children.
    • The study demonstrates a significant difference in CSF hypoxanthine levels between children with convulsions and those with aseptic meningitis.
    • Temporal analysis in rabbits supports the role of oxypurines as indicators of seizure-related metabolic disturbances.

    Related Experiment Videos