Isolation of glycogen from buffalo liver

The glycogen has been isolated from buffalo (Bos Bubalus bubalis) liver, purified several-fold, and characterized to compare with rabbit and oyster glycogen. Once-purified buffalo glycogen has been found to contain 1.3% protein, 0.16% nitrogen, 0.69% phosphorous, no lipids, and nucleic acids sufficient to cause absorption at 260 mu. The buffalo glycogen may be used as a potential substitute for rabbit and oyster glycogen after two-or three- purifications and a treatment with DEAE-cellulose.