Testing of aggregation measurement techniques for intramembranous particles

Under various physiological and nonphysiological conditions, the intramembranous particles, as seen by freeze-fracture electron microscopy, may be in various degrees of aggregation. To compare various schemes for the measurement of the degree of aggregation, a computer program has been used to generate simulated aggregations. A simple and adequate technique for quantifying the degree of aggregation, which is practical for the electron microscopist, is presented.