Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

A pericentric inversion in the mouse

M T Davisson, P A Poorman, T H Roderick

Cytogenetics and Cell Genetics

|January 1, 1981

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Nucleic acids and proteins of the nuclei of Paramecium.

Journal of morphology·2014

Same author

Mitotic chromosome preparations from mouse cells for karyotyping.

Current protocols in human genetics·2008

Same author

Two mouse retinal degenerations caused by missense mutations in the beta-subunit of rod cGMP phosphodiesterase gene.

Vision research·2007

Same author

Positional cloning, association analysis and expression studies provide convergent evidence that the cadherin gene FAT contains a bipolar disorder susceptibility allele.

Molecular psychiatry·2006

Same author

Mouse models of ocular diseases.

Visual neuroscience·2005

Same author

Report on the 'Expert Workshop on the Biology of Chromosome 21: towards gene-phenotype correlations in Down syndrome', held June 11-14, 2004, Washington D.C.

Cytogenetic and genome research·2005

Same journal

Gene mapping in marsupials and monotremes. II. Assignments to the X chromosome of dasyurid marsupials.

Cytogenetics and cell genetics·2012

Same journal

Assignment of the mouse Pcolce2 gene, which encodes procollagen C-proteinase enhancer protein 2, to chromosome 9 and localization of PCOLCE2 to human chromosome 3q23.

Cytogenetics and cell genetics·2002

Same journal

Assignment of the mouse Vegfb gene to mouse chromosome 19 B by in situ hybridization.

Cytogenetics and cell genetics·2002

Same journal

The mouse orthologue of the human ionotropic glutamate receptor-like gene (GRINL1A) maps(1) to mouse chromosome 9.

Cytogenetics and cell genetics·2002

Same journal

Assignment of GRINL1B, a glutamate receptor-like processed gene, to human chromosome 4q12 by in situ hybridization.

Cytogenetics and cell genetics·2002

Same journal

Assignment of progesterone receptor (PGR) to canine chromosome band 21q1.2 by in situ hybridization.

Cytogenetics and cell genetics·2002

See all related articles

A pericentric inversion in a Robertsonian chromosome (Rb4Bnr) was identified using chromosome banding and synaptonemal complex (SC) analysis. This chromosomal rearrangement is linked to reduced fertility in carriers.

Area of Science:

Cytogenetics
Reproductive Biology
Molecular Genetics

Background:

Robertsonian chromosomes can undergo rearrangements like pericentric inversions.
Understanding chromosomal abnormalities is crucial for reproductive health.
Synaptonemal complex (SC) analysis provides detailed insights into meiotic chromosome behavior.

Purpose of the Study:

To identify and characterize a pericentric inversion in a Robertsonian chromosome.
To investigate the meiotic behavior and synaptic adjustment of the inverted chromosome.
To determine the potential relationship between the inversion and reduced fertility.

Main Methods:

Chromosome banding techniques for initial identification of the Robertsonian chromosome (Rb4Bnr).
Synaptonemal complex (SC) analysis to visualize and map the inversion.

Related Experiment Videos

Analysis of SC inversion loops to determine breakpoint locations and meiotic progression.

Main Results:

A pericentric inversion, designated In(11.13LS)29Rk, was identified and characterized.
Breakpoints were mapped to 0.20 in the short arm and 0.54 in the long arm of the chromosome.
Inhibition of homologous synapsis and subsequent heterologous SC formation were observed during meiosis.

Conclusions:

The identified pericentric inversion significantly impacts homologous chromosome pairing during meiosis.
Synaptic adjustment mechanisms lead to heterologous SC formation in the presence of the inversion.
The chromosomal rearrangement is strongly implicated as the cause of reduced fertility in affected individuals.