Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The look-up table: a classifier for cell sorters

G J Koper, J G Christiaanse

    Cytometry
    |May 1, 1981
    PubMed
    Summary
    This summary is machine-generated.

    A novel look-up table classifier improves flow cytometry cell sorting. This method enhances cell cluster identification by matching user-defined regions to cell outlines, outperforming conventional circuitry.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    On the difference in ionization properties between planar interfaces and linear polyelectrolytes.

    Proceedings of the National Academy of Sciences of the United States of America·1997
    Same author

    Measurement of single-cell DNA synthesis by pokeweed mitogen-stimulated mononuclear cells with combined light and scanning electron microscopy.

    Immunology·1986
    Same author

    Flow cytometry of reticulocytes applied to clinical hematology.

    Blood·1983
    Same author

    An epiiluminator/detector unit permitting arc lamp illumination for fluorescence activated cell sorters.

    Cytometry·1982
    Same author

    The look-up table: a logarithmic converter for cell sorters.

    Cytometry·1981
    Same author

    Flow cytometry of human reticulocytes based on RNA fluorescence.

    Cytometry·1981
    Same journal

    Measurement of the distribution of red blood cell deformability using an automated rheoscope.

    Cytometry·2002
    Same journal

    Flow cytometric-based isolation of nucleated erythroid cells during maturation: an approach to cell surface antigen studies.

    Cytometry·2002
    Same journal

    Flow cytometric immunophenotyping test for staging/monitoring neuroblastoma patients.

    Cytometry·2002
    Same journal

    Determination of bronchoalveolar lavage leukocyte populations by flow cytometry in patients investigated for respiratory disease.

    Cytometry·2002
    Same journal

    Multicenter clinical experience with flow cytometric method for fetomaternal hemorrhage detection.

    Cytometry·2002
    Same journal

    Separation of live cells in different phases of the cell cycle for gene expression analysis.

    Cytometry·2002
    See all related articles

    Area of Science:

    • Biotechnology
    • Cell Biology
    • Analytical Chemistry

    Background:

    • Traditional flow sorter classifiers often use simplistic decision circuitry.
    • Existing methods may struggle with complex cell cluster shapes in multi-dimensional data.

    Purpose of the Study:

    • To introduce and experimentally validate a look-up table classifier for flow cell sorters.
    • To improve the accuracy of cell sorting by better matching classifier windows to cell cluster outlines.

    Main Methods:

    • An experimental setup integrating a microcomputer-controlled look-up table classifier with a commercial cell sorter was developed.
    • Look-up table contents were derived from user-defined regions of interest on bivariate flow data displayed visually.
    • The classifier was designed to match elliptic or ovoid shapes characteristic of cell clusters.

    Related Experiment Videos

    Main Results:

    • The look-up table classifier demonstrated improved matching of sorting windows to cell cluster contours.
    • This approach offers a more precise method for cell identification and sorting compared to conventional circuitry.
    • The system allows user-defined region designation for tailored sorting strategies.

    Conclusions:

    • Look-up table classifiers represent a significant advancement in flow cytometry cell sorting technology.
    • This user-centric approach enhances sorting accuracy by adapting to the natural shapes of cell populations.
    • The described method provides a more effective alternative to conventional flow sorter decision-making processes.