On the mechanism of intercellular adhesion

Using a 2 X 2 design, the collection rates of cells from radioactively labelled single-cell suspension on cell-coated collecting surfaces were tested for a possible H-2 effect on intercellular adhesiveness. In five donor-host combinations differing in the H-2 complex, no straight advantage of syngeneic over allogeneic relationship could unequivocally be proved. Trypsin was deliberately omitted in preparing the single-cell suspension; it was shown that the cell suspension processed in this way affected the mechanism of intercellular adhesiveness to a degree at least comparable to the role played by the properties of the collecting layer cells.