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Pulmonary polyamine permeability factor

J M Gleisner, L M Patt, C A Ramthun

    Inflammation
    |June 1, 1981
    PubMed
    Summary
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    Low-molecular-weight factors, identified as spermine and spermidine, increase microcirculation permeability in rats. These polyamines may enhance vascular permeability in lung tissue by releasing histamine.

    Area of Science:

    • Biochemistry
    • Physiology
    • Pharmacology

    Background:

    • Acid extracts of calf lung contain factors that affect microcirculation.
    • These factors are present in low levels in aqueous extracts.

    Purpose of the Study:

    • To identify and characterize low-molecular-weight factors in calf lung extracts that increase vascular permeability.
    • To investigate the role of these factors in histamine release and vascular permeability.

    Main Methods:

    • Gel filtration and ion-exchange chromatography for purification.
    • High-voltage paper electrophoresis to identify active compounds.
    • Assay of "blueing" reaction in rat skin to measure permeability.
    • Inhibition studies using pepstatin and pyrilamine maleate.

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    Main Results:

    • Two active compounds were isolated and identified as spermine and spermidine.
    • Both isolated and authentic spermine and spermidine increased vascular permeability.
    • Permeability activity was inhibited by pepstatin and pyrilamine maleate.

    Conclusions:

    • Spermine and spermidine are key polyamines that increase microvascular permeability.
    • Increased extracellular polyamine levels due to tissue damage may trigger histamine release, increasing lung vascular permeability.