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Solution structural studies of the Ag(I)-DNA complex

N Dattagupta, D M Crothers

    Nucleic Acids Research
    |June 25, 1981
    PubMed
    Summary
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    Silver ions induce DNA structural changes, altering DNA length and base pair orientation. These findings reveal silver-DNA interactions and DNA

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Structural Biology

    Background:

    • DNA exhibits various structural forms, including the B-form, which features propeller-twisted base pairs.
    • Metal ions, such as silver ions (Ag+), can interact with DNA and potentially alter its structure.
    • Ethidium bromide is a common intercalating agent used to study DNA structure and binding interactions.

    Purpose of the Study:

    • To investigate the structural consequences of silver ion binding to DNA.
    • To characterize the distinct complexes formed between silver ions and DNA.
    • To elucidate the role of silver ions in modulating DNA conformation and base pair geometry.

    Main Methods:

    • Equilibrium dialysis was employed to study the binding of silver ions to DNA.

    Related Experiment Videos

  • Electric dichroism was utilized to probe the structural changes and orientation of DNA and intercalated ethidium.
  • Analysis of DNA length changes and ethidium binding cooperativity.

    • Main Results:

    • Two strong complexes (Type I and Type II) of silver ions with DNA were identified.
    • Formation of the Type I complex resulted in a 9% decrease in DNA length and ethidium intercalation perpendicular to the helix axis.
    • Formation of the Type II complex restored the original DNA length, with ethidium becoming tilted relative to the helix axis.
    • Ethidium binding exhibited mild cooperativity in both Type I and Type II silver-DNA complexes.

    Conclusions:

    • Silver ions induce cooperative structural transitions in DNA, switching between conformations with flat and propeller-twisted base pairs.
    • The Type I complex involves a transition to a structure with flat base pairs, while the Type II complex reverts to propeller-twisted base pairs.
    • These findings provide insights into the sequence-dependent binding of silver ions and their impact on DNA structural dynamics.