A computer program for mathematical treatment of data in radioimmunoassay

A mathematical analysis of counting data obtained in hormone plasmatic radioimmunoassay is presented. A log-normal Galton distribution of the dose-effect type is assumed for the fitting of the experimental data. The special adopted procedure makes it possible to reproduce the total range of the sigmoid curve and not only the rectilinear part of it. Experimental data with iterations and the proper weights of the iterated counting rates are automatically taken into account and the statistical errors of the standard curve and of the sample's dose evaluated from it are given by the code. A large number of analyses have been performed in order to test the affidability of the method and to explain the validity of the chemical-physical parameters of the calculated distribution. Some selected results are presented and a full description of the mathematical formalism is given.