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Cell labelling with colloidal substances in whole blood
European Journal of Nuclear Medicine
|January 1, 1981
Summary
This study presents a new method for labeling white blood cells (leukocytes) using technetium-99m colloid. This technique effectively identifies inflammation in patients, aiding in the diagnosis of abdominal abscesses and infected endoprostheses.
Area of Science:
- Nuclear Medicine
- Immunology
- Medical Diagnostics
Background:
- Accurate localization of infection is crucial for effective patient management.
- Current diagnostic methods may have limitations in identifying deep-seated or localized infections.
Purpose of the Study:
- To describe a novel method for labeling leukocytes with 99mTc-colloid for infection imaging.
- To evaluate the efficiency and in vivo stability of the labeled leukocytes.
- To assess the clinical utility of this method in diagnosing abdominal abscesses and septic loosening of endoprostheses.
Main Methods:
- Leukocyte labeling using 99mTc-colloid in whole blood samples.
- Phagocytosed colloid is solubilized with Na-citrate for reinjection.
- Labeling efficiency measured at 80-90% with 50% cell viability after 3 hours.
Main Results:
- High labeling efficiency (80-90%) achieved.
- Significant portion (at least 50%) of labeled leukocytes remained in circulation after 3 hours.
- Clinical studies in 32 patients with suspected abdominal abscesses and 42 with septic endoprostheses demonstrated the method's efficacy.
Conclusions:
- The described 99mTc-colloid labeling method for leukocytes is efficient and stable.
- Labeled leukocytes are effective in localizing inflammatory foci.
- This technique shows promise for diagnosing abdominal abscesses and septic loosening of endoprostheses.