Ultra trace determination of furazolidone in turkey tissues by liquid partitioning and high performance liquid...

A simple and sensitive procedure is presented for the determination of furazolidone in turkey tissues, using liquid partitioning followed by high performance liquid chromatography (HPLC). Fat, liver, kidney, skin, and muscle tissues are ground with methylene chloride in a Polytron homogenizer, followed by solvent removal, partitioning in hexane-0.01M acetic acid, and back-partitioning the 0.01M acetic acid with methylene chloride. The determination by HPLC used a reverse phase Ultrasphere-ODS 5 micrometer column. The method is sensitive to 0.5 ppb, with a standard deviation of 6.39% at the 2 ppb fortification level. Recovery from fortified tissues averaged 84% from samples fortified with 0.5-10 ppb furazolidone. An alternative cleanup procedure using a Sep-Pak C18 cartridge is also presented.