Related Experiment Video
Updated: Aug 17, 2026

Preparation of Synaptoneurosomes from Mouse Cortex using a Discontinuous Percoll-Sucrose Density Gradient
Published on: September 17, 2011
Abstract:
The preparation of sn-glycero-1-phosphocholine is described. 2,3-O-Isopropylidene-sn-glycerol is condensed first with the 1,2-dimethylethenylene phosphorochloridate in the presence of triethylamine to yield the cyclic phosphotriester derivative. Choline p-toluenesulfonate, as the second alcohol, is condensed with this intermediate to yield the phosphotriester bearing the 1-methyl-acetonyl blocking group. This is removed under basic conditions to afford the 2,3-O-isopropylidene-sn-glycerol-1-phosphocholine in 57% yield following silicic acid column chromatography. The acetone blocking group is then removed with aqueous HCl (pH 2.3) to give pure sn-glycero-1-phosphocholine.
Related Concept Videos
Prochirality
SN1 Reaction: Stereochemistry
In the first step of an SN1 reaction, the bond between the electrophilic carbon and the leaving group ionizes to generate the carbocation intermediate. The second step of the mechanism is the nucleophilic attack.
In the formed carbocation, the positively charged carbon is sp2 hybridized with a trigonal planar geometry. As all the three substituents lie on the same plane, a plane of symmetry for the...
Oligosaccharide Assembly
Multiple sugar molecules that may or may...
Overview of Fatty Acid Metabolism
Fatty acids are catabolized in a process called beta-oxidation, which takes place in the matrix of the mitochondria and converts their fatty acid chains into two-carbon units of acetyl groups. The acetyl...
Synthesis of Phosphatidylcholine in the ER Membrane
The major components of all eukaryotic cell...
Cholinergic Neurons: Neurotransmission

