[Changes in kidney enzyme activity in temporary ischemia of the extremities]

Histoenzymological changes in the kidneys of 49 dogs with temporary ischemia of posterior extremities and acute renal insufficiency were studied. The results demonstrate a definite relationship between the intensity of histoenzymological changes in the kidneys and the duration of temporary ischemia of the extremities. With 12-hour ischemia of the extremities, epitheliocytes of proximal tubules showed a decreased activity of aerobic oxidation enzymes and increased activity of glycolytic enzymes, epitheliocytes of the distal tubules showed a decreased activity of the enzymes under study. After revascularization the level of metabolism in the examined parts of nephrons decreased as confirmed by the results of quantitative assays.