Inhibition of collagen cross-linking: a new approach to ocular scarring

A method for the production of alkali-induced conjunctival scarring in rabbits with minimal corneal involvement is presented. The lathyritic agent Beta-aminopropionitrile (BAPN), an inhibitor of collagen cross-linking, was applied topically for a period of 21 days after induction of injury. There was a statistically significant (p less than .025) increase in both the interpalpebral fissure length and width of scarred treated animals compared with the scarred control group. BAPN did not affect the interpalpebral fissure measurements of unscarred rabbit eyes. No clinical adverse effects were observed after the treatment period. Histological evaluation of the treated scar tissue was consistent with lathyrism.