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Attempted direct visualization of negatively stained amplified immune complex of synaptic acetylcholinesterase using
Histochemistry
|January 1, 1981
Abstract:
An immunocytochemical method is proposed for the localization of synaptic acetylcholinesterase (AChE) on ultrathin frozen sections of the electric organ of the electric eel. The immune complex formed is amplified by a non-specific "sandwich" technique and visualized by negative staining. Definite white spots on synaptic cleft seem to correspond to basal lamina AChE molecules.