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Increased hemoglobin-oxygen affinity does not decrease skeletal muscle oxygen consumption

B K Ross, M P Hlastala

    Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology
    |October 1, 1981
    PubMed
    Summary
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    Hemoglobin-oxygen affinity (HOA) changes do not significantly impact skeletal muscle oxygen consumption (VO2). Stored blood reduced VO2, but this effect was unrelated to HOA, suggesting other factors are involved.

    Area of Science:

    • Physiology
    • Biochemistry

    Background:

    • Hemoglobin-oxygen affinity (HOA) influences oxygen delivery to tissues.
    • Previous studies suggested HOA is critical for skeletal muscle oxygen consumption (VO2).

    Purpose of the Study:

    • To reevaluate the importance of HOA in skeletal muscle VO2.
    • To investigate factors other than HOA affecting VO2 in stored blood.

    Main Methods:

    • Isolated canine gracilis muscle model used.
    • HOA manipulated by refrigerated storage and chemical treatment (sodium metabisulfite, sodium cyanate).
    • VO2, venous O2 partial pressure (PvO2), and arterial oxygen saturation measured under constant flow.

    Main Results:

    • Stored blood significantly reduced VO2 (to 80% of control) without changing PvO2.

    Related Experiment Videos

  • Blood treated with sodium metabisulfite or sodium cyanate did not impair VO2.
  • Carbamylated blood (increased HOA) showed VO2 identical to fresh blood.
  • Stored blood demonstrated reduced VO2 across various oxygen saturations, independent of HOA.

    • Conclusions:

    • The position of the oxygen dissociation curve (HOA) may be less critical for skeletal muscle oxygen delivery than previously thought.
    • Reduced VO2 with stored blood is likely due to factors other than altered HOA.