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Cryo-electron Microscopy Specimen Preparation By Means Of a Focused Ion Beam
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Published on: July 26, 2014

Chromoblastomycosis: cure by cryosurgery

R R Lubritz, J E Spence

    International Journal of Dermatology
    |December 1, 1978
    PubMed

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    Cryo-electron Microscopy01:28

    Cryo-electron Microscopy

    Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...