Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Quantitative mass spectrometry

S P Markey

    Biomedical Mass Spectrometry
    |September 1, 1981
    PubMed
    Summary

    Quantitative mass spectrometry enables precise measurement of substances in biological samples. This study highlights its application in high-sensitivity analysis and establishing definitive reference measurements, addressing data conflicts.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Gas chromatographic-mass spectrometric method for the assessment of oxidative damage to double-stranded dna by quantification of thymine glycol residues.

    Journal of the American Society for Mass Spectrometry·2013
    Same author

    Characterization of anthraquinone-2-carbonyl chloride as an alcohol derivatization reagent for negative ion chemical ionization mass spectrometry.

    Journal of the American Society for Mass Spectrometry·2013
    Same author

    A low voltage ion transport system for external ionization fourier transform mass spectrometry.

    Journal of the American Society for Mass Spectrometry·2013
    Same author

    Pentafluorobenzyl chloroformate derivatization for enhancement of detection of amino acids or alcohols by electron capture negative ion chemical ionization mass spectrometry.

    Journal of the American Society for Mass Spectrometry·2013
    Same author

    Liquid chromatography particle beam-mass spectrometry with massive cluster impact.

    Journal of the American Society for Mass Spectrometry·2013
    Same author

    Proteomic analysis of nuclei dissected from fixed rat brain tissue using expression microdissection.

    Analytical chemistry·2013

    Area of Science:

    • Analytical Chemistry
    • Biochemistry
    • Biophysics

    Background:

    • Quantitative analysis relies on mass spectrometry to measure analytes against internal standards.
    • Ensuring signal specificity in complex biological matrices requires advanced analytical methods.
    • Challenges arise in achieving high sensitivity (parts per billion and lower) due to instrument limitations and data interpretation debates.

    Purpose of the Study:

    • To demonstrate successful high-sensitivity (parts per trillion) plasma analysis of melatonin using quantitative mass spectrometry.
    • To critically compare this sensitive analysis with existing conflicting data.
    • To examine the application of quantitative mass spectrometry in establishing definitive reference measurements with minimized errors.

    Main Methods:

    • Combination of mass spectrometry with chromatographic separations (gas or liquid).
    • Utilizing selective chemical derivatization for enhanced specificity and sensitivity.
    • Rigorous error minimization protocols for definitive reference measurements.

    Main Results:

    • Successful high-sensitivity (parts per trillion) plasma analysis of melatonin was achieved.
    • Conflicting analytical data for melatonin were critically compared.
    • Conflicting results from two laboratories on definitive serum cholesterol measurements were analyzed.

    Conclusions:

    • Quantitative mass spectrometry, particularly when coupled with chromatography and derivatization, is effective for high-sensitivity analysis.
    • The method's rigor is crucial for establishing definitive reference measurements and resolving data discrepancies.
    • Careful analytical method selection and validation are essential for reliable quantitative data in complex biological matrices.

    Related Experiment Videos