Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Combined high performance liquid chromatography mass spectrometry

Biomedical Mass Spectrometry

|September 1, 1981

Summary

Combined liquid chromatography-mass spectrometry is a powerful technique for identifying and quantifying chemicals, especially those not suitable for gas chromatography. This method enhances analysis of complex samples like natural products, drugs, and pesticides.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Multichemical defense of plant bugHotea gambiae (westwood) (Heteroptera: Scutelleridae) : Sesquiterpenoids from abdominal gland in larvae.

Journal of chemical ecology·2013

Same author

Multichemical defense of plant bugHotea gambiae (Westwood) (Heteroptera: Scutelleridae): (E)-2-hexenol from abdominal gland in adults.

Journal of chemical ecology·2013

Same author

Quantitative determination and structural characterization of isoflavones in nutrition supplements by liquid chromatography-mass spectrometry.

Journal of chromatography. A·2005

Same author

Comparison of high-speed counter-current chromatography instruments for the separation of the extracts of the seeds of Oroxylum indicum.

Journal of chromatography. A·2005

Same author

Urinary modified nucleosides as tumor markers.

Nucleosides, nucleotides & nucleic acids·2003

Same author

Analysis of urinary nucleosides. II. Comparison of mass spectrometric methods for the analysis of urinary nucleosides.

Rapid communications in mass spectrometry : RCM·2001

Area of Science:

Analytical Chemistry
Biochemistry
Environmental Science

Background:

Gas chromatography-mass spectrometry (GC-MS) offers high sensitivity and specificity but is limited by sample volatility and thermal stability.
Many organic compounds, including natural products and synthetic chemicals, are not amenable to GC analysis.
Chemical modification can be used but is not universally applicable for GC analysis.

Purpose of the Study:

To introduce and discuss combined liquid chromatography-mass spectrometry (LC-MS) as a solution for analyzing thermally labile or non-volatile compounds.
To highlight the advantages of LC-MS in overcoming the limitations of GC-MS.
To demonstrate the capabilities of LC-MS in identifying and quantifying chemicals in complex matrices.

Main Methods:

Utilizing high-performance liquid chromatography (HPLC) for separation of compounds that are not amenable to gas chromatography.
Coupling HPLC with mass spectrometry (MS) to leverage the sensitivity and specificity of MS detection.
Applying LC-MS to analyze natural compounds, drugs, and pesticides.

Main Results:

LC-MS enables the analysis of compounds unsuitable for GC due to thermal lability or low volatility.
The technique successfully identifies and quantifies target chemicals within complex mixtures.
Demonstrated applications include the analysis of natural products, pharmaceuticals, and pesticides.

Conclusions:

Combined liquid chromatography-mass spectrometry is a versatile and powerful technique for chemical analysis.
LC-MS significantly expands the scope of compounds that can be analyzed with high sensitivity and specificity.
This method is crucial for research in natural products, drug discovery, and environmental monitoring.

Related Experiment Videos