Hydroxyproline-rich protein in the capsule of a strain of Staphylococcus aureus

A protein fraction containing a high amount of hydroxyproline was found in the capsule of the Smith diffuse strain (encapsulated) when compared with that of dialysate medium. This content varied depending upon temperature, oxygen supply, sodium chloride, and carbohydrates. The hydroxyproline content of the single proteinaceous fraction, obtained by Sephadex G-200 column chromatography, was one fourth of the total amino acid composition.