In vitro perfusion technique for investigations on the intestinal transport of water soluble substances

A new method for the in vitro perfusion of isolated intestinal segments of laboratory animals is described. Everted intestinal segments are used in an "open perfusion system," and are incubated in a buffer solution containing the substrate to be absorbed as a radiolabeled compound. The solutes absorbed are removed from the serosal side in a buffer solution that is collected by a fraction collector. The technique permits studies on the mechanism and the kinetics of the intestinal transport of water soluble substances.