Improved red blood cell storage using optional additive systems (OAS) containing adenine, glucose and...

Area of Science:

• Biochemistry
• Hematology
• Biotechnology

Background:

• Red blood cell (RBC) storage is limited by metabolic degradation.
• Maintaining RBC viability and function during storage is crucial for transfusion medicine.

Purpose of the Study:

• To enhance RBC storage and function using an optional additive system (OAS).
• To investigate the impact of adenine, glucose, and ascorbate-2-phosphate (AsP) on RBC viability and oxygen-offloading capacity.

Main Methods:

• RBCs were collected using citrate-phosphate-dextrose (CPD) or CPD-adenine.
• RBCs were treated with OAS solutions containing varying combinations of adenine, glucose, and AsP.
• RBC viability was assessed by adenosine triphosphate (ATP) concentrations.
• RBC oxygen-offloading capacity was evaluated by 2,3-diphosphoglycerate (2,3-DPG) levels.

Main Results:

• Adenine and extra glucose maintained RBC viability, evidenced by stable ATP concentrations.
• Ascorbate-2-phosphate (AsP) effectively preserved 2,3-DPG levels, enhancing oxygen-offloading characteristics.
• RBCs stored with specific OAS formulations (CPD + adenine, glucose, AsP or CPD-adenine + AsP) were maintained for at least 42 days.

Conclusions:

• The developed OAS formulations significantly extend RBC storage duration.
• The addition of adenine, glucose, and AsP to RBCs improves storage stability and functional capacity.
• This approach offers a promising strategy for improving blood product utilization and patient outcomes.