[Production of primary cells cultures by a semi-industrial technic. II. Use of active trypsin solutions for effective...

For effective cell yields in semi-industrial production of primary cell suspensions, the use of trypsin solutions with high enzyme activity has been proved to be an essential requirement. Enzyme solutions with a constant high activity may be obtained by using an optimal filtration method. Filtration results using the deep and membrane filtration method to sterilize trypsin solutions are demonstrated and discussed. A number of trypsin samples taken from the "Leidholdt-Firm" were tested concerning cell yields in the so-called 'Biomix-Procedure'. A correlation could be shown to exist between enzyme activity and cell yield. The so tested trypsin-L-samples out of the years 1976/78 were proved to be first-rate preparations for routine production of primary piglet and calf kidney cell cultures.