Related Experiment Video
Updated: Sep 25, 2026

Test Samples for Optimizing STORM Super-Resolution Microscopy
Published on: September 6, 2013
Abstract:
Lack of resolution (unsharpness) can reduce contrast in diagnostic radiography if the proper conditions of magnification and unsharpness are met. To describe this phenomenon, a modification of the contrast reduction factor (CRF) was introduced which used the response function of a semi-opaque edge to predict contrast reduction for small bar-shaped objects. To predict CRF, unsharpness is employed as a single-term description of resolution and is obtained experimentally from the edge response function. The unsharpness term is defined as the distance over which the response goes from 16.5% to 83.5% of the maximum. Measured and predicted CRFs were compared and the CRF concept was found to be an excellent predictor of contrast reduction. The individual components of unsharpness were determined experimentally and the sum-of-squares rule predicted adequately their combination. Three methods to measure unsharpness were compared: (a) the ICRU prescription using pinhole radiographs of the focal spot, (b) one-dimensional integration of the focal-spot pinhole radiograph, and (c) the unsharpness term produced by a semi-opaque edge. The latter two were measured using a microdensitometer.
Related Concept Videos
NMR Spectrometers: Resolution and Error Correction
Imaging Biological Samples with Optical Microscopy
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Phase Contrast and Differential Interference Contrast Microscopy
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Super-resolution Fluorescence Microscopy
Downsampling
The Fourier transform of the decimated sequence reveals a combination of scaled and shifted versions of the original spectrum. This...
Block Diagram Reduction
The first step in this process is the identification and relocation of a branch point. A branch point, where a...

