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Haemiglobin cyanide: molar lineic absorbance and stability constant
Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Fur Klinische Chemie Und Klinische Biochemie
|December 1, 1981
Summary
The molar absorbance of haemiglobin cyanide was determined. The stability constant for haemiglobin cyanide was calculated using titration data.
Area of Science:
- Biochemistry
- Analytical Chemistry
Background:
- Haemiglobin cyanide (HiCN) is a complex formed between haemiglobin and cyanide.
- Understanding the formation and stability of HiCN is crucial for biochemical assays and toxicological studies.
Purpose of the Study:
- To determine the molar lineic absorbance of haemiglobin cyanide.
- To calculate the stability constant (equilibrium association constant) of haemiglobin cyanide.
Main Methods:
- Direct titration of haemiglobin with cyanide.
- Analysis of the titration curve to determine absorbance and stability constant.
Main Results:
- The molar lineic absorbance (εHiCN) of haemiglobin cyanide was found to be 1101 m²·mol⁻¹ at 540 nm.
- The stability constant (KHiCN) for haemiglobin cyanide was calculated as 1.5 x 10⁶ L/mol.
Conclusions:
- The study provides key quantitative data on haemiglobin cyanide formation.
- These values are essential for accurate spectrophotometric quantification of haemiglobin and cyanide interactions.