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Measurement of Heme Synthesis Levels in Mammalian Cells
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Published on: July 9, 2015

A multi-wavelength spectrophotometric method for the simultaneous determination of five haemoglobin derivatives

A Zwart, A Buursma, E J van Kampen

    Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Fur Klinische Chemie Und Klinische Biochemie
    |July 1, 1981
    PubMed

    A method is described by which the concentration of deoxyhaemoglobin, oxyhaemoglobin, carboxyhaemoglobin, haemoglobin and sulfhaemoglobin in a human blood sample is determined by passing the haemolysate without air contact through a coarse filter and subsequently measuring the absorbance at lambda = 500, 569, 577, 620 and 760 nm. The ensuing set of equations is solved by matrix calculation with the aid of a simple computer program. The method has been tested by comparing it with conventional methods for the determination of the various haemoglobin derivatives separately.

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