[Comparative studies of the lipoprotein complexes in bull seminal fluid diluted with different media]

Electrophoresis on celogel and lipid staining with Fettrot 7 were employed to investigate the lipid complexes in the media Hagase Niva, Triladil, Leciphos, and GH22L (after Kichev). Studied were likewise the changes that these lipid complexes underwent when using them to dilute bull semen to a varying extent. It was found that the lipid complexes in these media changed their electrophoretic characteristic and percent distribution following dilution. At 1 + 1 dilution all media were shown to build up a new lipoid complex having a protein base and comprising 84 to 96 per cent of the lipid components in the samples. On the basis of Kichev's phenomenon of complex forming and building new biologic molecules the possibility is discussed of the practical use of these investigations in the cryoprotection of bull semen.